R&D Manufacturing offer

In gene therapy development, assays are often performed on immortalized cells and further translated to primary cells and tissues before in vivo experiments take place.

Manufacturing dyptique

Different products depending on your application

Flash Therapeutics offers two lentiviral vector delivery system and two qualities of vectors depending on your applications:

1. Integrative lentiviral vector (iLV) :

  • Start quality (>107 IG/ml) for Research-grade vectors 
    • Very high level of transduction on permissive and “hard-to-transfect” immortalized cell lines

The Start batch is dedicated to screen and select the best vector candidates. 

  • Premium quality (>109 IG/ml) for Research-grade to GMP-grade vectors 
    • Very high level of transduction on primary & stem cells 
    • No cell damage thanks to the low impurity content 
    • Safe and clinically  suitable delivery without inducing toxicity or immunogenicity 
    • In vivo genetic transfer

The Premium batch is dedicated to in vitro or ex vivo approaches, in vivo gene therapy, transgenesis or direct tissue injection experiments.

 

Start and Premium quality lentiviral vector specifications
Bacth type Volume (mL) Titre (IG/mL) PP/IG
Start 1-5 >107 <1000
Premium 1-60 >109 <300

Titre (IG/mL) : Number of lentiviral functional particles leading to integration of vector DNA into the host genome.
PP/IG ratio : Gives a measure of the lentiviral batch quality by discriminating the non functional physical particles (PP) from the functional particles.

 

2. Biological RNA delivery technology (non-integrative LentiFlash) :

  • Start quality (>109 PP/ml) for Research-grade vectors 
    • Very high level of transduction on permissive and “hard-to-transfect” immortalized cell lines

 

  • Premium quality (>1011 PP/ml) for Research-grade to GMP-grade vectors 
    • Very high level of transduction on primary & stem cells 
    • No cell damage thanks to the high purity level  
    • In vivo, in vitro and ex vivo cell transduction

The Premium batch is dedicated to in vitro or ex vivo approaches, in vivo gene therapy, or direct tissue injection experiments.

 

Start and Premium quality lentiviral vector specifications
Bacth type Volume (mL) Titre (PP/mL)
Start 1-5 >109
Premium 1-60 >1011

Titre (PP/mL) : Number of physical p24 particles per mL.
 

High quality lentiviral solutions since 2005

With a strong know-how in lentiviral vector production and a robust bioproduction process, Flash Therapeutics has produced over 9000 lentiviral vector batches for scientists all over the world.

Contact Adel to design your project


Adel Houhou


Adel Houhou - Global Account Manager

Linkedin profile


Feel free to contact him to design your project