10 best practices for a good transduction efficiency
2016-08-04Cell line generation
- Electroporation,
- Lipid or chemical transfection,
- Viral vectors…
A wide range of tools is available for gene transfer experiments. Among these, the lentiviral vector technology has proven to be an effective tool with significant benefits. This is mainly due to its efficiency, its gentleness with delicate cells, the stability of expression and the shortened time lapse of experiments. Unfortunately, results are not always living up to expectations. To prevent failure, several parameters must be taken into account. We describe in this article 10 best practices to follow for a good transduction efficiency.
- Lipid or chemical transfection,
- Viral vectors…
A wide range of tools is available for gene transfer experiments. Among these, the lentiviral vector technology has proven to be an effective tool with significant benefits. This is mainly due to its efficiency, its gentleness with delicate cells, the stability of expression and the shortened time lapse of experiments. Unfortunately, results are not always living up to expectations. To prevent failure, several parameters must be taken into account. We describe in this article 10 best practices to follow for a good transduction efficiency.
