Poster CRISPR congress Berlin 2016

Vectalys attended the CRISPR congress Berlin 2016 to present an innovative RNA delivery particle named LentiFlash. This particle is based on a new chimeric bacteriophage-based lentiviral platform allowing transient RNA delivery into target cells without any genomic scar.

Various speakers invited to the congress discussed the CRISPR-Cas9 system and its use for future clinical applications. Key point often brought up was the delivery tool to carry the CRISPR-Cas 9 system in a safe and efficient way. Because the CRISPR-Cas 9 stable expression may result in off-target effects, many scientists choose transient RNA delivery tools.

The information gathered at the congress strengthened our view presented in the poster: our non-integrative RNA delivery particle Lentiflash differs from all existing systems and meets all the requirements for an efficient and safe delivery of the CRISPR-Cas 9 system.

Gene knock-out with LentiFlash carrying CRISPR-Cas9 system:

Highly concentrated and purified at Vectalys, our new particle is able to deliver non-viral coding or non-coding RNA at high efficiency. Our poster shows the ability of the LentiFlash particle to deliver the CRISPR-Cas9 system in different types of cells without affecting viability, including human primary T lymphocytes, preserving the original phenotype of the cells. Three points are approached:

• LentiFlash: a new tool for efficient and transient CRISPR-Cas9 delivery
• Highly efficient disruption of PD-1 in human primary T-cells using CRISPR-Cas9
• LentiFlash: benefits from lentiviral vector properties without genomic trace

To discover our poster  click here .