LentiFlash® Technology

LentiFlash® is constructed using a bacteriophage coat protein and its cognate 19-nt stem loop, to replace the natural lentiviral Psi packaging sequence, in order to achieve active mRNA packaging into the lentiviral particles (Prel et al, Mol Ther 2015 DOI10.1038/mtm.2015.39 ).

LentiFlash® suspensions are produced using Flash Therapeutics process, as for conventional lentiviral vectors, also resulting in highly pure and concentrated vectors.

As it is a biological RNA transfer technology, LentiFlash® particles cannot be titrated in IG/mL. Therefore LentiFlash® are titrated using a p24 method, and titers are provided in µg p24 / mL.

Safe and efficient RNA delivery

LentiFlash® particles are VSV-G pseudotyped and highly purified. These two criteria make LentiFlash® very efficient for the transfer of biologically active RNAs into various cell types including immortalized, primary, stem and iPS cells.
LentiFlash® is a safe RNA delivery technology since it preserves the original cell phenotype, cell proliferation and differenciation ability and a full cell viability.

Transient and fast expression

LentiFlash® delivers RNAs directly into the cell cytoplasm, rendering RNAs rapidly bioavailable, which lead to a high, fast (as early as 4h post-transduction)  expression of your sequence of interest. These assets make LentiFlash® particularly suitable to carry antigens, reprogrammation factors or CRISPR/Cas9 system into cells of interest, while preserving original cell phenotype and viability. 

Short-term and transient expression using LentiFlash®, varying according to the half-life of the protein of interest
To learn more, please see our Nature publishing group paper.

Key applications

Thanks to the high and short-term expression of transferred RNAs, LentiFlash® is particularly suitable for:

• Vaccination and Immunotherapy approaches
• Gene editing
• Regenerative medicine