LentiFlash® is a biological RNA delivery technology
LentiFlash®, a non-integrative lentiviral particle dedicated to cell and gene therapy applications, allows:
- A safe and efficient RNA delivery technology
- A delivery of multiple RNA species
- A fast expression of your sequence(s) of interest
- A transient RNA expression
LentiFlash® : RNA-delivery system based on a bacteriophage-lentivirus chimera
LentiFlash® is constructed using a bacteriophage coat protein and its cognate 19-nt stem loop, to replace the natural lentiviral Psi packaging sequence, in order to achieve active mRNA packaging into the lentiviral particles (Prel et al, Mol Ther 2015 DOI10.1038/mtm.2015.39 ).
LentiFlash® suspensions are produced using Flash Therapeutics process, as for conventional lentiviral vectors, also resulting in highly pure and concentrated vectors.
As it is a biological RNA transfer technology, LentiFlash® particles cannot be titrated in IG/mL. Therefore LentiFlash® are titrated using a p24 method, and titers are provided in µg p24 / mL.
Safe and efficient RNA delivery
LentiFlash® particles are VSV-G pseudotyped and highly purified. These two criteria make LentiFlash® very efficient for the transfer of biologically active RNAs into various cell types including immortalized, primary, stem and iPS cells.
LentiFlash® is a safe RNA delivery technology since it preserves the original cell phenotype, cell proliferation and differenciation ability and a full cell viability.
Transient and fast expression
LentiFlash® delivers RNAs directly into the cell cytoplasm, rendering RNAs rapidly bioavailable, which lead to a high, fast (as early as 4h post-transduction) expression of your sequence of interest. These assets make LentiFlash® particularly suitable to carry antigens, reprogrammation factors or CRISPR/Cas9 system into cells of interest, while preserving original cell phenotype and viability.
Short-term and transient expression using LentiFlash®, varying according to the half-life of the protein of interest
To learn more, please see our Nature publishing group paper.
Thanks to the high and short-term expression of transferred RNAs, LentiFlash® is particularly suitable for:
- Vaccination and Immunotherapy approaches
- Gene editing
- Regenerative medicine